Amplification:Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 were amplified from human UNC93B1 (BC025669.1) and iRhom2 cDNA (NM_024599.2; Origene (SC122961)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 iRhom2 Δ201-300 , and FRMD8-iRhom2 Δ300 were amplified from human UNC93B1 (BC025669.1), human iRhom2 cDNA (NM_024599.2; Origene (SC122961)) and human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
Polymerase Chain Reaction:Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 were amplified from human UNC93B1 (BC025669.1) and iRhom2 cDNA (NM_024599.2; Origene (SC122961)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 iRhom2 Δ201-300 , and FRMD8-iRhom2 Δ300 were amplified from human UNC93B1 (BC025669.1), human iRhom2 cDNA (NM_024599.2; Origene (SC122961)) and human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
Clone Assay:Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 were amplified from human UNC93B1 (BC025669.1) and iRhom2 cDNA (NM_024599.2; Origene (SC122961)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
Article Title: FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Article Snippet: .. Human UNC93B1, human iRhom2 WT , iRhom2 Δ100 , iRhom2 Δ200 , iRhom2 Δ300 , iRhom2 Δ382 iRhom2 Δ201-300 , and FRMD8-iRhom2 Δ300 were amplified from human UNC93B1 (BC025669.1), human iRhom2 cDNA (NM_024599.2; Origene (SC122961)) and human FRMD8 cDNA (NM_031904; Addgene (SC107202)) by PCR and cloned with an C-terminal 3xHA tag into the lentiviral vector pLEX.puro using Gibson assembly (New England Biolabs) following the manufacturer’s instructions. .. C-terminal V5-tagged FRMD8 (FRMD8-V5) was amplified from human FRMD8 cDNA (Addgene (SC107202)) by PCR and cloned into pcDNA3.1(+) using Gibson assembly.
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